Occurrence and antimicrobial-resistant Salmonella serovars isolated from turkey carcasses and giblets in Meknès-Morocco

The present study was conducted to determine the Salmonella prevalence, the plasmids, the serotypes involved and antimicrobial susceptibility patterns of Salmonella isolates recovered from the raw meat of the turkey in various outlets in Meknes. Between October 2011 and October 2012, a total of 192 samples of turkey meat (included 48 breasts, 48 legs, 48 gizzards and 48 livers) were collected every ten days from retail outlets in Meknès. Among these, 48 were from popular market, 48 from artisanal slaughterhouses, 48 from poulterers’ shops and 48 from a supermarket at Meknes, Morocco. Of the total of 192 samples examined, 24.5% were contaminated with Salmonella. Out of the total 48 samples analysed from popular market, 40.42% proved to be Salmonella positive whereas from 48 samples obtained from traditional slaughterhouses and 48 from poulterers’shops 29.87 and 17% contained Salmonella, respectively. The sizes of the plasmids obtained ranged from 1.8 to 100 kb. All Salmonella isolates were tested for their susceptibility to 13 selected antimicrobial agents by the agar diffusion method. The highest percentage of resistance was found to the following antimicrobial agents: Bacitracin (97.8%), amoxicillin (61.7%), streptomycin (44.6%), triméthoprime (34%), nalidixic acid and colistin (19.1%), ciprofloxacin (17%). On the other hand, 97.8% of isolates were found to be resistant to one or more of the antibiotics tested. Only one strain (S. Agona), the resistance profile (amoxicillin, chloramphenicol, streptomycin, bacitracin, cefotaxime, ceftazidime, céftriaxone, colistine) is an ESBL with a minimum inhibitory concentration for ceftriaxone 16 μg/ml.